Fig. 2: Inhibition of a-syn aggregation by nasal brushing matrix: re-testing of the negative nasal brushing samples (n = 50) after spiking with PD brain lysate. | npj Parkinson's Disease

Fig. 2: Inhibition of a-syn aggregation by nasal brushing matrix: re-testing of the negative nasal brushing samples (n = 50) after spiking with PD brain lysate.

From: Combining skin and olfactory α-synuclein seed amplification assays (SAA)—towards biomarker-driven phenotyping in synucleinopathies

Fig. 2

a The lag times in the SAA seeded by both nasal brushings and brain lysate (n = 50) compared to the same brain lysate tested alone (n = 15 separate assays), each dot represents the mean of 4 replicates. Mean values (±SD) are plotted in gray for each group. b Examples of lag-phase prolongation and change in ThT curve morphology in the PD brain lysate aggregation caused by the addition of some of the nasal brushings. See Supplementary Fig. 2 for further examples. c Mean (±SD) of Hb concentration in the PD nasal brushings at the assay-relevant dilution (1:20) grouped based on the SAA outcome. d Correlation of Hb concentration with the lag time of samples spiked with PD lysate. The typical range of the lag times in the brain lysate alone is shaded.

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