Fig. 6: Mice injected with α-synuclein fibrils develop cognitive dysfunction.

A Schematic representation of the Barnes maze platform in which the target and reversal target are 180° apart. Quantification of the average latency across acquisition trials at (B) 1 MPI, (C) 3 MPI, and (D) 6 MPI. Data are expressed as boxplots depicting the median, interquartile range, and individual data points for the average latency of control and fibril-injected mice (n = 10–11 animals/group). **p < 0.01 or ***p < 0.001 by unpaired Student’s t test. E Spaghetti plot depicting the average latency of mice at 1, 3, and 6 MPI. Each line represents the performance trajectory of an individual mouse. Quantification of performance trajectory from (F) 1 MPI to 3 MPI and (G) 3 MPI to 6 MPI. Data are expressed as boxplots depicting the individual data points for the net change in average latency of each group from 1 MPI to 3 MPI and from 3 MPI to 6 MPI. **p < 0.01 by unpaired Student’s t test. H Boxplots depicting individual data points for the percentage of time in which mice occupied the target quadrant during the probe session. I Boxplots depicting the individual data points for the average speed during the probe session. J Boxplots depicting individual data points for the average latency of each group during the reversal segment of the Barnes maze. **p < 0.01 or ***p < 0.001 by two-way ANOVA with Bonferroni’s multiple comparisons test, as indicated. K Boxplots depicting individual data points for the percentage of time in which mice occupied the target quadrant during the probe session during the reversal segment of the Barnes maze. *p < 0.05 by unpaired Student’s t test.