Fig. 2: Acetylated α-Tubulin distribution in the fibres of the central and peripheral nervous system. | npj Parkinson's Disease

Fig. 2: Acetylated α-Tubulin distribution in the fibres of the central and peripheral nervous system.

From: Linking acetylated α-Tubulin redistribution to α-Synuclein pathology in brain of Parkinson’s disease patients

Fig. 2

a, b Acetylated α-Tubulin is localised in nigro-striatal fibres of putamen but not in neuronal cell bodies in controls (a–a’), whereas it is enriched mainly in neuronal cell bodies in PD patients (b, b’; black arrow). BF: bundle of fibres Scale bar, 100 μm. c–e In controls, acetylated α-Tubulin positive axons in putamen run parallel to each other (c, c’; dashed square: 2x magnified) while they are less dense in PD samples (d, d’; dashed square: 2x magnified). Nuclei are counterstained with Hoechst. The graphs show the percentage of the area covered by acetylated α-Tubulin (e; Ctrl, N = 3 vs PD, N = 4) in putamen BF. Scale bar, 20 μm. Data in graphs are reported as mean ± standard deviation. Mann-Whitney test, *p < 0.05. f–h Acetylated α-Tubulin localisation (f, g) within PGP 9.5 positive fibres (f”, g”) around sweat glands both in control subjects (f–f”) and PD patients (g–g”). Merge in (f’, g’). Nuclei are counterstained with Hoechst. The graph ((h) Ctrl, N = 9 vs PD, N = 12) indicates the percentage of the area covered by acetylated α-Tubulin in PGP 9.5 positive fibres. f, g Scale bar, 10 μm. f’, f”, g’, g”: Scale bar, 20 μm. Data in graphs are reported as mean ± standard deviation. Mann-Whitney test, *p < 0.05.

Back to article page