Fig. 3: Representative confocal immunofluorescence images comparing α-Syn aggregate morphologies and epitope-specific α-Syn antibody immunoreactivities between pathologically significant regions of human brains with PD.

Yellow arrowheads indicate epitope-specific N-terminus immunoreactivity (AA 34–45), magenta arrowheads indicate epitope-specific pS129 immunoreactivity, and white arrowheads indicate epitope-specific C-terminus immunoreactivity (AA 118–123). Epitope-specific immunoreactivities and net overlapping immunoreactivities are depicted by the scaled percentage quantification bar. Quantifications recorded as percentage of total α-Syn immunolabelling area (yellow, epitope-specific N-terminus; magenta, epitope-specific pS129; white, epitope-specific C-terminus; green, overlapping immunolabelling). All region-specific confocal images were acquired from the same relative anatomical locations (Fig. 2a). MTG, middle temporal gyrus; MFG, middle frontal gyrus; AA, amino acids. Scale bar, 10 μm.