Fig. 2: Differential transcript expression (DTE) analysis results.

Volcano plots of differential transcript expression (DTE) in a males and b females with an FDR threshold of <0.2. c During testing, we identified 2534 differentially expressed transcripts by PFF treatment in males and 1419 differentially expressed transcripts in females (FDR < 0.2). d Venn diagram of differential transcript expression (DTE) results in male and female rats. A total of 326 transcripts were identified as differentially expressed in both sexes. e Dotplots of GO term enrichment testing for the up- and downregulated genes that overlapped in males and females. GO term enrichment was performed using a hypergeometric test in the clusterProfiler R package. Enriched GO biological process (GOBP) terms for upregulated genes are on the left, enriched GOBP for downregulated genes are on the right. GeneRatio refers to the ratio of genes in the GOBP term to the total number of input genes, and qvalue refers to the Benjamini-Hochberg-adjusted p-value. GeneRatio is represented by the size of dots in the plot, and q-value is represented by color.