Fig. 4: Idiopathic PD patients exhibit a circular RNA-specific reduction in expression.
From: Early-stage idiopathic Parkinson’s disease is associated with reduced circular RNA expression
a Proportion of loci increased in PD relative to controls based on differential expression testing of back-spliced junctions (BSJs), forward-spliced junctions (FSJ), genes which harbour a BSJ (BSJ hosts) and all genes (Gene). P-values obtained from a two-sided exact binomial test corrected for multiple testing (Bonferroni correction, four tests). Error bars show the 95% CI of the imbalance estimate. b Comparing the expression of all BSJs included in differential expression testing between PD and controls in PPMI and ICICLE-PD. P-values are derived from a Wilcoxon rank-sum test comparing BSJ expression between PD and controls. c Correlation between BSJ and FSJ expression fold changes in both PPMI and ICICLE-PD. The correlation coefficients are reported as Spearman’s rho. Density plots show the distribution of the fold changes for each respective junction type. d Comparison of fold changes between BSJs and FSJs in PPMI and ICICLE-PD. P-values derived from a Wilcoxon rank-sum test comparing fold changes between each junction type. e, f Plots showing the normalised expression of the genes RNASEL and EIF2AK2 (which encodes PKR) in PD and controls from PPMI and ICICLE-PD. Log2 fold changes and P-values were obtained from the results of testing the differential expression of genes.
