Fig. 2: TauK18- and TauK19-SAA analyses at ankle and cervical sites in disease sub-groups.
From: Tau seeding activity in skin biopsy differentiates tauopathies from synucleinopathies
Kinetic curves of TauK18 and TauK19 seeding activity in the ankle (A, K) and cervical (F, P) sites, respectively. Each dot represents the mean (±SE) of ThT fluorescence intensity of all samples tested per group against time. The dotted line indicates the selected threshold at 10.000 arbitrary units (AU). B, G, L, Q Scatter plot of final ThT fluorescence intensities: dots represent the mean values of the final ThT fluorescence intensity for individual patients and bars the mean of the group. C, H, M, R Box and Whisker Plot showing the Lag time defined as the reaction time required to pass the threshold of 10.000 AU. The dotted line shows the selected time points (7 h for TauK18 or 18 h for TauK19) to define the positive and negative subjects. D, I, N, S Box and Whisker Plot showing the T50 defined as the reaction time required to reach 50% of the maximum fluorescence detected. E, J, O, T Seeding score defined as the number of replicates out of three that cross the threshold of 10.000 AU at 7 h. Each dot represents a patient and bars the mean of the group.
