Fig. 6: Spontaneous and glutamatergic modulator induced neuronal activity in conventional networks after PFF treatment.

a Illustration of the percent changes in neuronal activity, with the dotted line representing baseline activity recorded prior to PFF treatment. b Pairwise analysis depicting mean firing rates at 13 dpt compared to baseline values recorded in the same well before PFF or PBS vehicle treatment. c A schematic representing serial stimulation with glutamatergic modulators, namely, NMDA in conjunction with Glycine, D-AP5, AMPA, and CNQX. d Outputs to each glutamatergic modulator were presented as percent changes to the previously calculated neuronal activity in the same well (dotted lines) for controls and e for PFF treated networks. f 3D representation of multiparametric analysis presented with the first three principal components (PC1-3) and calculated intersection percentages between the surfaces of control and PFF treated network values. Each data point in the images represented values obtained from a conventional MEA well. The number of MEA wells (n) included to analysis range from 14 to 16 per group in different time points. Whiskers and horizontal lines in quantifications indicate the range and the median values, respectively. p values were calculated with the Wilcoxon Signed Ranked test for pairwise comparisons to baseline recordings (b) or to conditions prior to stimulations (d, e) (*p < 0.05, **p < 0.01 and ***p < 0.001). The illustrations were created using Biorender.com.