Table 1 Reagents and peptides used in the study
From: CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease
REAGENT | SOURCE | CATALOG NUMBER |
|---|---|---|
Thalidomide | Sigma | Cat# 50-35-1 |
IPTG | Sigma | Cat# 367-93-1 |
ThT | Sigma | Cat# 9041-08-1 |
Lipofectamine 2000 | Invitrogen | Cat# 11668019 |
PMSF | Sigma | Cat# 10837091001 |
Protease inhibitor cocktail | Calbiochem | Cat# 535140 |
Normal goat serum | Abcam | Cat# Ab7481 |
Aquamount | Thermoscientific | Cat# TA-125-AM |
Hoescht Dye | Abcam | Cat# 145597 |
FSC22 clear | Leica | Cat# 3801480 |
DAB substrate | Vector lab. | Cat# SK-4100 |
ImmPACT NovaRED® | Vector lab. | Cat# SK-4805 |
ProteinG Sepharose | GE Healthcare | Cat# 17-0618-01 |
Metal Affinity resin | Clontech | Cat# 635502 |
Flag M2 agarose beads | Sigma | Cat# A2220 |
MPTP | Sigma | Cat# 506382 |
ChariotTM | Activemotif | Cat# 30025 |
Recombinant protein α-SYN | This Study | NA |
Recombinant protein DNAJB1 | This Study | NA |
Recombinant protein DNAJB6 | This Study | NA |
Recombinant protein DNAJA1 | This Study | NA |
Recombinant protein Hsp70 | This Study | NA |
Recombinant protein APG2 | This Study | NA |
