Fig. 7: Identification of downstream mediators of ONECUT2’s effect on GCase activity. | npj Parkinson's Disease

Fig. 7: Identification of downstream mediators of ONECUT2’s effect on GCase activity.

From: Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2

Fig. 7

a Transcriptomic profiling of selected DEGs following after CRISPRa of ONECUT2 in LN-229L444P cells (N = 3 replicates). Enzymatic activity in cell lysates following activation of top 30 DEGs in LN-229WT (b) or LN-229L444P (c) (N = 7 to 10). d Lysosomal enzymatic activity assessed with LysoFQ-GBA1 following CRISPRa of PLEKHG4 or PLEKHG4B in LN-229WT and LN-229L444P cells (N = 3). Median values of the NT controls set as 100%. Solid lines: medians and quartiles. Unpaired t-test.

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