Table 2 List of antibodies for immunoblotting

From: Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2

Target

Manufacturer

Dilution

GCase

G4171 (Sigma-Aldrich), produced in rabbot

1: 4000

Vinculin

ab91459 (Abcam) produced in rabbit

1:5000

Beta-Actin (HRP-coupled)

PA1-183-HRP produced in mouse; Invitrogen

1: 10,000

USF2

clone 5E9 H00007392-M01 (Abnova) produced in rabbit

1: 1000

MITF

Clone D5G7V #12590 (CellSignaling), produced in rabbit

75.9 ug/mL at 1:1000

ONECUT2

21916-1-AP (Proteintech), produced in rabbit

0.6 mg/mL at a 1:750 dilution