Table 1 Primary and secondary antibodies used for immunofluorescence
Primary antibodies | Cat. Number | RRID | Host | Dilution | Manufacturer |
|---|---|---|---|---|---|
Tyrosine hydroxylase | #MAB318 | AB_2201528 | Mouse | 1:1000 | Millipore |
IBA1 | #019-19741 | AB_839504 | Rabbit | 1:800 | Wako |
DOPA Decarboxylase | #GTX134053 | AB_2887192 | Rabbit | 1:800 | Gene Tex |
CD3 | #ab16669 | AB_443425 | Rabbit | 1:200 | Abcam |
Secondary antibodies | Cat. Number | IgG | Dilution | ||
Alexa Fluor 488 donkey | #A21202 | AB_141607 | Mouse | 1:200 | Thermo Fisher Scientific |
Alexa Fluor 555 donkey | #A31572 | AB_162543 | Rabbit | 1:200 | Thermo Fisher Scientific |