Fig. 1: The nigro-striatal pathway presents diverse ECS properties and matrix complexity. | npj Parkinson's Disease

Fig. 1: The nigro-striatal pathway presents diverse ECS properties and matrix complexity.

From: Intracellular α-synuclein assemblies are sufficient to alter nanoscale diffusion in the striatal extracellular space

Fig. 1: The nigro-striatal pathway presents diverse ECS properties and matrix complexity.

a SWCNT tracking and analysis experimental workflow. SWCNTs were administered in vivo into the lateral ventricles and allowed to diffuse into the brain for 1 h. Acute brain slices were prepared in ice-cold NMDG-based solution and imaged in aCSF at 35 °C under carbogen bubbling. SWCNTs were excited using an 845 nm laser. Post hoc analysis permitted the super-localization and trajectory reconstruction. Each trajectory provided a local map and distribution of values of local diffusivities (Dinst/Dref) and local channel widths. These maps reveal a heterogeneous ECS in the nigro-striatal pathway (SNc: substantia nigra pars compacta, SNr: substantia nigra pars reticulata, Str: striatum). b Distributions of Dinst/Dref values (one-way ANOVA F (2, 77608) = 157.4, p value < 0.0001) and c Distributions of local ECS widths (nm) values (one-way ANOVA F (2, 77608) = 97.41, p value < 0.0001). SNc presents a significantly different diffusion regime and local sizes than SNr and Str. d Scatter plots depicting the relationship between channel width and instantaneous diffusion coefficient for each data point (n = 4 mice SNc and SNr. n = 10 mice Str). Created in BioRender. Bezard, E. (2023) BioRender.com/i55x918.

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