Fig. 3: Decreased TH+ fiber densities from 6-OHDA (20 µg; 2 weeks post infusion).

Clusters with decreased TH-IF from 6-OHDA after FDR correction and validation by fiber density measurements are shown (quantification for additional valid clusters is in Fig. S5). Data are split into columns based on the comparison of post hoc voxel-wise t-tests. Left: decreased TH-IF in the ipsilateral (ipsi) hemisphere of 6-OHDA-treated mice compared to their contralateral (contra) hemisphere. Middle: decreased TH-IF in the ipsilateral hemisphere of 6-OHDA-treated mice compared to the ipsilateral hemisphere of vehicle-infused mice. Right: decreased TH-IF in the contralateral hemisphere of 6-OHDA-treated mice compared to the contralateral hemisphere of vehicle-infused mice. A 3D brain models of valid clusters. B Sunburst plots summarize regional volumes of valid clusters across levels of the ABA hierarchy. C Quantification of TH+ fiber density for each cluster. Fiber density was defined as: (volume of segmented voxels/total cluster volume) × 100. Clusters were numbered in order of size but organized based on anatomy. The largest region of each cluster is depicted by its abbreviated name. Valid cluster locations, volumes, and regional compositions are summarized in Table S3. It also contains raw fiber densities for all clusters. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001. Abbreviations are defined in the ABA and Table S3. Other abbreviations include: d = dorsal; l = lateral; p = primary or posterior; r = rostral; s = secondary or supplemental; v = ventral.