Fig. 5: Striatal tyrosine hydroxylase (TH) levels following infusion of 5, 10, or 20 µg of 6-OHDA are reduced in male C57BL/6 mice.

A, E Representative western blot membranes probed for TH (green, molecular weight ≈58 kDa) and α-tubulin (red, molecular weight ≈ 50 kDa). TH protein levels serve as a proxy for striatal dopaminergic lesions. B–D Quantification of TH protein levels in male cohorts for 5 µg, 10 µg, and 20 µg dosages of 6-OHDA, respectively. F–H Quantification of TH protein levels in female cohorts for 5 µg, 10 µg, and 20 µg dosages of 6-OHDA, respectively. *p < 0.05, **p < 0.01, and ****p < 0.0001. The same vehicle group was used as a control across all 6-OHDA dosages. V = vehicle, S = sham. 19.5 µg of protein was loaded per well. Data are reported as mean ± SEM. Group sizes: Male cohort: Vehicle group: n = 6; 6-OHDA groups: 5 µg day 1 n = 7, day 7 n = 8, and day 14 n = 6; 10 µg day 1 n = 8, day 7 n = 8, and day 14 n = 5; 20 µg day 1 n = 5, day 7 n = 5, and day 14 n = 6. Female cohort: n = 5 for each group. Refer to Figs. S6 and S7 for full gel images and data normalized to the loading control α-tubulin. Excluded outliers (ROUT test) are shown as clear symbols with a red outline.