Fig. 8: Striatal infusion of 20 µg of 6-OHDA altered inflammatory markers at the infusion site and in the SNpc.

A Three days after 6-OHDA infusion, inflammatory marker proteins in striatal lysates were analyzed using Western blotting. Each well was loaded with 19.5 µg of total protein. Densitometric quantification of B CD68, C Iba1, D LAMP-1, and E GFAP for vehicle and 6-OHDA groups, with expression normalized to GAPDH or α-tubulin (two-tailed unpaired t-tests). F–H A Luminex immune monitoring panel quantified protein levels of 48 cytokines and chemokines in lysates from the SNpc at days 1, 3, and 7 post-infusion. The expression of immune proteins is represented by log₂-fold changes in the mean fluorescence intensity (MFI) relative to vehicle groups (two-tailed unpaired t-tests). Red and blue bars represent significant upregulation and downregulation, respectively. I–S For chemokines/cytokines with a change in expression, we plotted their baseline level (collected from 6 sham animals; see Methods) and their expression in 6-OHDA- and vehicle-treated groups at each time point (pair-wise Bonferroni-corrected comparisons). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data are reported as mean ± SEM. Western blotting cohort: vehicle n = 8, 6-OHDA n = 7. An excluded outlier (ROUT test) is shown as a clear symbol with a black outline. Multiplex cytokine cohorts: day 1 vehicle n = 6, 6-OHDA n = 8; day 3 vehicle n = 6, 6-OHDA n = 6; day 7 vehicle n = 6, 6-OHDA n = 8.