Fig. 5: Complement C4 exacerbates inflammation and neuronal damage in vivo. | npj Parkinson's Disease

Fig. 5: Complement C4 exacerbates inflammation and neuronal damage in vivo.

From: Complement C4 exacerbates astrocyte-mediated neuroinflammation and promotes α-synuclein pathology in Parkinson’s disease

Fig. 5

A Mice were stereotactically injected with α-syn PFF, C4, α-syn PFF + C4, or PBS into the right striatum. Behavioral tests, including pole climbing, rotarod performance, and hanging test, were conducted after 3 months to assess motor function. B TH immunohistochemistry was performed on the substantia nigra and striatum. Scale bar = 200 μm. C Statistical analysis showed increased dopaminergic neuronal loss in the substantia nigra-striatum pathway with α-syn PFF + C4 treatment. D qPCR analysis of midbrain tissue showed elevated mRNA levels of IL-1β, IL-6, and TNF-α with α-syn PFF + C4 treatment. E Immunoblotting demonstrated higher protein levels of IL-1β, IL-6, and TNF-α, as well as reduced TH levels in midbrain tissue following α-syn PFF + C4 injection. F Quantification of TH, IL-1β, IL-6, and TNF-α protein levels for each condition in (E).

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