Fig. 2: Altered network of PV-INs rs-FC in 6-OHDA mice. | npj Parkinson's Disease

Fig. 2: Altered network of PV-INs rs-FC in 6-OHDA mice.

From: Parkinsonism disrupts cortical function by dysregulating oscillatory, network and synaptic activity of parvalbumin positive interneurons

Fig. 2

A Experimental timeline for PV-INs longitudinal WF calcium imaging. B Representative WF field-of-view. Colored squares represent cortical ROI parcellation based on the Allen Brain Atlas. White dots indicate Bregma (top) and Lambda (bottom). Scale bar = 1 mm. C Representative 2-s sequences of resting state (RS) cortical activity at 14, 21 and 28 days post lesion (DPL) in control (CNT, top) and 6-OHDA group (bottom). Temporal sequences were randomly selected from the imaging dataset. D Dynamics of the average functional connectivity PV network (Pearson’s correlation) over the days 14, 21, 28 DPL for CNT (bottom right) and 6-OHDA (top left). E FC differences matrix between CNT and 6-OHDA over the days, calculated by subtracting the average FC of the CNT group from that of the 6-OHDA group. Red = 6-OHDA hyperconnectivity; blue = 6-OHDA hypoconnectivity. F Network diagram of statistically significant FC alterations at 28 DPL (left). Red/blue lines represent significant hyper/ hypo-correlations of 6-OHDA compared with CNT, respectively. The bar plot (right) shows the number of significant FC (hypo left, hyper right) for each cortical area (p < 0.05, NBS). G Box plot showing the mean global signal PV-FC in the CNT group compared to the 6-OHDA group at 14,21 and 28 DPL.). H Box plot showing the mean inter-hemispheric PV-FC in the CNT group compared to the 6-OHDA group at 14, 21 and 28 DPL. PV-FC in the posterior area of the secondary motor cortex between CNT and 6-OHDA group at 14, 21 and 28 DPL in the left hemisphere (I) and right hemisphere (J). C Comparison of PV-FC in the anterior area of the primary motor cortex between CNT and 6-OHDA group at 14, 21 and 28 DPL in the left hemisphere (K) and right hemisphere (L). M, N Comparison of PV-FC in the barrelfield cortex between CNT and 6-OHDA group at 14, 21 and 28 DPL in the left hemisphere and right hemisphere. In blue, CNT n = 5 and in orange, 6-OHDA n = 4. Data are expressed as mean ± Standard Error of Mean. *p ≤ 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. See also Supplementary Fig. 3 and Supplementary Table 2.

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