Table 1 Reagents List
From: Parkinson’s paradox: alpha-synuclein’s selective strike on SNc dopamine neurons over VTA
General reagents | Concentration | Vendor | Catalog number |
|---|---|---|---|
Paraformaldehyde | 4% | Electron Microscopy Sciences | 157-4-100 |
Preformed Fibrils | 2 µg/µL | Provided by Giasson Lab | N/A |
AAV1-TH-hαSyn | 4.92 × 1013 U/µL | Provided by Giasson Lab | N/A |
AAV-FLEx-GCaMP8f | 1.1 × 1013 | Addgene | 162379-AAV5 |
Blocking solution 1 | |||
Triton X-100 | 0.3% | Thermo Fisher Scientific | 9002-93-1 |
Bovine Serum Albumin | 5% | Sigma | A2153-100G |
Fetal Bovine Serum | 5% | GeminiBio | 100-106 |
PBS | 1X | prepared on-site | N/A |
Blocking solution 2 | |||
Bovine Serum Albumin | 5% | Sigma | A2153-100G |
Fetal Bovine Serum | 5% | GeminiBio | 100-106 |
PBS | 1X | prepared on-site | N/A |
