Fig. 2: Synaptic protein density in cortical and limbic areas in the D-PD mouse model.
From: Early synaptic changes and reduced brain connectivity in PD-like mice with depressive phenotype
Male mice received 1 μl of AAV2/5-CBh-WPRE3 construct to drive expression of h-α-Syn (AAV-h-α-Syn) or empty AAV2/5-CBh-WPRE3 vector containing non-coding (null) stuffer DNA (AAV-EV) into dorsal raphe nucleus (DR) and were euthanized at 4 and 8 weeks (W) post-injection. A, C, E Representative coronal brain image showing SV2A-ir, synaptophysin (SYP)-ir, and MAP-2-ir, respectively in the medial prefrontal cortex (mPFC), cingulate cortex (Cg), caudate-putamen (CPu), and hippocampus (HPC) of mice at different time points after AAV2/5 injection. Scale bar: low: 200 μm. B, D, F Bar chart showing the density of SV2A, SYP, and MAP-2 markers in the brain areas analyzed: prelimbic cortex (PrL), infralimbic cortex (IL), Cg, CPu, and different HPC subfields (n = 4–10 mice/group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus AAV-EV or AAV-h-α-Syn 4 W mice). Values are presented as mean ± SEM.
