Fig. 3: Single-cycle kinetic experiment with α-syn and immobilized SVD-1 and SVD-1a and control peptides.
From: Direct disassembly of α-syn preformed fibrils into α-syn monomers by an all-D-peptide
A SVD-1 (left) and SVD-1a (right) were immobilized on a carboxyl dextran matrix via amino coupling until saturation was reached (CMD200M, Xantec, GE). α-Syn was injected for 100 s at 30 µl/min in PBS 7.4 in a serial dilution ranging from 30 to 500 nM, followed by a dissociation time of 60 or 30 min, respectively. The experiment was performed as an individual measurement. The interaction kinetics were fitted with a 1:1 kinetic interaction model: SVD-1: KD: 880 pM, kon: 6.56 × 104 M−1 s−1, koff: 5.78 s−1 × 10−5; SVD-1a: KD: 100 pM, kon: 3.13 × 105 M−1 s−1, koff: 3.13 × 10−5 s−1. The non-referenced signal of the active and referenced surface is shown in Supplementary Fig. 7. B SVD-1_scrambled (left) and SVD-1_scrambled+5r (right) were immobilized on a carboxyldextran matrix via amino coupling until saturation was reached (CMD200M, Xantec, GE). Full-length α-syn was injected for 100 s at 30 µl/min in PBS 7.4 in a serial dilution ranging from 15 to 250 nM, followed by a dissociation time of 60 min. Y-axis scaling was adjusted to (A).
