Fig. 2: Intestinal pathological phenotypes of WT, LRRK2R1627P and LRRK2−/− rats at different ages. | npj Parkinson's Disease

Fig. 2: Intestinal pathological phenotypes of WT, LRRK2R1627P and LRRK2−/− rats at different ages.

From: LRRK2R1627P mutation amplifies environmental risk factors induced chronic inflammation and α-synuclein aggregation in the gut of rats

Fig. 2: Intestinal pathological phenotypes of WT, LRRK2R1627P and LRRK2−/− rats at different ages.

a Measurement of small intestine length. Scale bar 5 cm, n = 8. Two-way ANOVA was used; interaction p < 0.0001. b Schematic diagram of the sampling site. The term “small intestine” used in this study refers to the proximal jejunum tissue. c HE staining images of the small intestine. Scale bar 100 μm, n = 4. d Measurement of villus height and crypt depth in the small intestine, n = 4. Two-way ANOVA was used; interaction p < 0.0001. e Immunohistochemical staining images of Ki67 in the small intestine, and the quantification of Ki67 positive cells in the crypts (determined based on the number of brown-stained cell nuclei in the crypts). Scale bar 100 μm, n = 4. Two-way ANOVA was used; interaction p < 0.01. f Immunohistochemical staining images of Cleaved-caspase 3 in the small intestine, and the quantification of Cleaved-caspase 3 positive cells in the villus. Arrows show the apoptotic cells. Scale bar 100 μm, n = 4. Two-way ANOVA was used; interaction p > 0.05. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01.

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