Fig. 1
Comparison of ESDMs and BMDMs. Stained cytospins of BMDMs (a) and ESDMs (b) with image analyses demonstrating that ESDMs are larger (c) (Scale bars 30 μM). Flow cytometry analyses of BMDMs (d) and ESDM (e) demonstrating a pure population in ESDMs. Images from live phagocytosis assay of BMDMs (g) and ESDMs (h) at 0 (I), 50 (II, 100 (III) and 150 (IV) minutes after the addition of Phrodo beads and quantification of rate of phagocytosis using Harmony image analysis software (i) (Scale bars 50 μM). See Supplementary Fig. S1 for details of the assay. [*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n = 3, c Unpaired t-test, f One-way ANOVA with Kruskal-Wallis test, i Two-way ANOVA with Sidak’s multiple comparison test]
