Fig. 1
From: Identification of regenerative roadblocks via repeat deployment of limb regeneration in axolotls
Regenerative decline after repeated amputation. Both forelimbs were amputated and allowed to fully regenerate. a Experimental overview. b Skeletal preparations of limbs following successive rounds of amputation. The limb in bottom right panel failed to regenerate beyond the plane of amputation (dashed line). c Representative examples of sibling control limb (top left panel) and limb that failed to regenerate after repeated amputation (bottom left panel). d Cumulative distribution plot of loss in ability to regenerate beyond the plane of amputation (right graph). Scale bars in b are 1 mm
