Fig. 2

Regenerative decline after repeated amputation is less severe if the plane of amputation is shifted distally along the axis of the limb. Both forelimbs were amputated for each animal and allowed to fully regenerate. a Experimental overview. b Representative images of axolotl limbs after 5 rounds of amputation at the same plane (left panels) or with serially distal planes of amputation (right panels). c Quantification of the number of limbs able to regenerate digits after each amputation round following repeated amputation in the same plane or repeated, progressively distal amputations. Asterisk (*) indicates p < 0.05 (Fisher’s Exact Test). All scale bars equal to 1 mm