Fig. 5

Amphiregulin is expressed during the wound healing stage of limb regeneration. In situ hybridization analyses of areg expression in wild-type juvenile axolotls; a–f are regenerating limb samples; g–h are flank skin wound samples. a Expression of areg at 3 h post-amputation. Arrowheads indicate expression of areg in the leading edge of the wound epidermis. Epi epidermis. b Higher magnification view of panel a. c Section stained with sense control probe at 3 h post-amputation. d Expression of amphiregulin at 12 h post-amputation. Arrowheads indicate expression of areg in the wound epidermis (we). e Tissue section stained with areg anti-sense in situ probe at 72 h post-amputation. f Tissue section stained with areg anti-sense in situ probe at 14 days post-amputation (medium bud blastema). g In situ hybridization showing areg expression in leading edge of wound epidermis at 6 h post-biopsy. h Higher magnification view of panel g. We wound epidermis, bl blastema. Images are representative of four biological replicates per time point. Scale bars are 100 µm