Fig. 4 | npj Regenerative Medicine

Fig. 4

From: Relative contributions of adipose-resident CD146+ pericytes and CD34+ adventitial progenitor cells in bone tissue engineering

Fig. 4

Bidirectional paracrine effects of CD146+ pericytes and CD34+ adventitial cells during osteogenic differentiation in non-contact co-culture in vitro. a Representative Alizarin Red S staining, whole well images after 14 days osteogenic differentiation. b Quantitative analysis of Alizarin Red S staining shown using absorbance at 548 nm. c Quantitative RT-PCR analysis of osteogenic gene markers (RUNX2, ALP, COL1A1) and angiogenesis-related gene markers (MCP1, IGFBP1, IGFBP2) among CD34+ adventitial cells with or without pericyte co-culture, day 7 of osteogenic differentiation. d Quantitative RT-PCR analysis of osteogenic and angiogenic gene markers among CD146+ pericytes with or without adventitial cell co-culture, day 7 of osteogenic differentiation. *P < 0.05. N = 3 wells per condition

Back to article page