Fig. 3
From: Encapsulation of macrophages enhances their retention and angiogenic potential
The effect of alginate encapsulation on the pro-angiogenic function of Tie2-iBMMs. a–d Representative light microscope images of HUVEC/fibroblast angiogenesis assays for HUVECs co-cultured with empty alginate capsules a, 100 ng/ml VEGF b, naked Tie2-iBMMs c or alginate encapsulated Tie2-iBMMs d. e HUVEC tubule area was compared (n = 6–12/group, *P = 0.05 **P = 0.01 ***P = 0.0001 by Kruskal Wallis test, error bars = s.d.). f Quantification of PlGF-2, VEGF, MMP9, IL-10 and IL-1β secreted by nTie2-iBMMs (green) and eTie2-iBMMs (grey) following in vitro culture for up to 21 days (n = 5/group, **P = 0.01, ***P = 0.001, ****P < 0.001 by two-way ANOVA and Bonferroni post-test, error bars = s.e.m.). g, h Quantification of Vegfa164 g and MCP-1 h secreted by stimulated Tie2-iBMMs using ELISA (n = 6/group, **P = 0.01 by Mann Whitney test, error bars = s.d.)
