Fig. 2: Characterisation of mobilised CFU-Fs. | npj Regenerative Medicine

Fig. 2: Characterisation of mobilised CFU-Fs.

From: Pharmacological tools to mobilise mesenchymal stromal cells into the blood promote bone formation after surgery

Fig. 2: Characterisation of mobilised CFU-Fs.The alternative text for this image may have been generated using AI.

Mice were pretreated with BRL37344 (β3) once daily for 4 days. One hour after the last injection, mice were administered AMD3100 and 1 h later blood was collected for analysis of circulating (a–c) CFU-Fs. a Photomicrograph image depicts a blood mobilised CFU-F colony stained with Giemsa. b Histograms of surface marker expression on culture-expanded CFU-F cells as determined by flow cytometry. Shaded histograms represent marker expression and open dashed-line histograms represent fluorescence minus one (FMO) controls. c Panel shows trilineage differentiation staining of FACS sorted CD45– culture-expanded CFU-Fs into osteocytes (Alizarin Red S), adipocytes (Oil Red) and chondrocytes (Alcian Blue). Scale bars, 100 µm. Experiment representative of n = 10 mice.

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