Table 1 Phenotypic qualities and chondrogenic differentiation potential of MSCs from different tissues.
Cell type | Species | Induction method | Analysis method | Comparison | Ref | ||
|---|---|---|---|---|---|---|---|
Type 1 | Type 2 | Type 3 | |||||
BMSCs | ADSCs |  | Human | Monolayer cultured and 3D pellet cultured in chondrogenic medium for 2 weeks | Cartilage related gene expression analysis, Safranin O staining, IHC staining for COL II | BMSCs =  ADSCs in monolayer, BMSCs >  ADSCs in 3D culture. | |
CD34−CD45− CD49d−CD73+CD90+CD105+ CD166+ BMSCs | CD34−CD45− CD49d−CD73+CD90+CD166+ADSCs |  | Human | 3D pellet cultured in chondrogenic medium for 4 weeks | GAG content, COL I, COL II, Sox9 genes expression, Safranin O staining, IHC staining for COL II, IF analysis for Sox-9. | BMSCs >  ADSCs | |
CD31−CD34− CD45− HLA-DR− CD29+CD44+CD90+CD105+HLA-ABC+ BMSCs | CD31−CD34− CD45− HLA-DR− CD29+CD44+CD90+CD105+HLA-ABC+ ADSCs |  | Human | 3D pellet cultured in chondrogenic medium for 3 weeks | IHC staining for COL II | BMSCs >  ADSCs | |
CD11b−CD45−CD44+CD90+ SDSCs | CD11b−CD45−CD44+ BMSCs | CD11b−CD45−CD44+ ADSCs | Canine | 3D pellet cultured in chondrogenic medium for 3 weeks | sGAG content | SDSCs =  BMSCs >  ADSCs | |
SDSCs | BMSCs | ADSCs | Porcine | Cells were seeded on hydrogels and cultured in chondrogenic medium for 7 weeks | sGAG and collagen content | SDSCs >  ADSCs >  BMSCs | |
CD11b−CD45−CD90+ SDSCs | CD11b−CD45−CD90+ BMSCs | CD11b−CD45−CD90+ ADSCs | Rat | 3D pellet cultured in chondrogenic medium for 3 weeks | Pellets weight, toluidine blue staining, COL II gene expression, chondroitin sulfate and hyaluronan content | SDSCs >  BMSCs >  ADSCs | |
CD34−CD45− HLA-DR− CD44+CD73+CD90+CD105+WJMSCs | CD34−CD45− HLA-DR− CD44+CD73+CD90+ BMSCs |  | Human | Cells were seeded on hydrogels and cultured in chondrogenic medium without added growth factor for 4 weeks | Cartilage-specific transcript expression analysis, Alcian blue staining, IF and IHC staining for COL II | WJMSCs >  BMSCs | |
WJMSCs | BMSCs |  | Human | Cells were seeded on PGA scaffolds and cultured in chondrogenic medium without added growth factor for 6 weeks | sGAG content, IHC staining for COL I and COL II | WJMSCs >  BMSCs | |
