Fig. 1: Differentiation of definitive endoderm (DE) cells on decellularized lung scaffolds resembles in situ epithelial differentiation of native mouse airways. | npj Regenerative Medicine

Fig. 1: Differentiation of definitive endoderm (DE) cells on decellularized lung scaffolds resembles in situ epithelial differentiation of native mouse airways.

From: TP63 basal cells are indispensable during endoderm differentiation into proximal airway cells on acellular lung scaffolds

Fig. 1

a Progression of airway development in murine lung visualized by SEM (top panel) and TEM (bottom panel). b Electron micrographs of DE-scaffolds at day 7 of culture. c Electron micrographs of adult native airway epithelia. d Electron micrographs of DE-scaffolds at day 21 of culture. e-left panel Electron micrographs and immuno-TEM of DE-scaffolds at days 14 of culture. e-right-panel Immunogold labelling for SCGB1A1 of club cells at day 21. SEM, scanning electron microscopy; TEM, transmission electron microscopy; Lu, lumen; ci, ciliated cell; se, secretory cell; ba, basal cell. Scale Bars: a SEM 5 μm, TEM 2 μm; b SEM-left 5 μm, TEM-left 2 μm, SEM-right 1 μm; TEM-right 500 nm; c, d SEM 5 μm, TEM 2 μm; e TEM 2 µm, Immuno TEM-right 250 nm.

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