Fig. 6: Transcriptome profiling of wildtype and TP63-deficient definitive endoderm (DE) cells seeded on decellularized lung scaffolds. | npj Regenerative Medicine

Fig. 6: Transcriptome profiling of wildtype and TP63-deficient definitive endoderm (DE) cells seeded on decellularized lung scaffolds.

From: TP63 basal cells are indispensable during endoderm differentiation into proximal airway cells on acellular lung scaffolds

Fig. 6

a Schematic of RNA-seq experiments. b Heat map of genes predominantly associated with endoderm, lung progenitor, basal and proximal (secretory and ciliated) airway cells, respectively; columns show averaged log expression between TP63wt and TRP63−/− DE-scaffolds per time point. c GSEA of DE cells for gene sets related to mesenchyme development and epithelial to mesenchymal transition in TP63−/− DE cells compared to wildtype DE cells. RNA sequencing was repeated 4 times using 4 separate wildtype and 3 separate TP63-deficient DE cell-scaffold cultures. Averaged fold change in (b) is expressed as log2.

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