Fig. 1: Isolation of chondrocytes from juvenile cartilage surgical discards and in vitro expansion of juvenile chondrocytes. | npj Regenerative Medicine

Fig. 1: Isolation of chondrocytes from juvenile cartilage surgical discards and in vitro expansion of juvenile chondrocytes.

From: Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment

Fig. 1

A Juvenile donor-derived cartilage tissues under stereomicroscope. Scale bar: 5 mm. B Safranin-O staining of cartilage tissue. Scale bar: 500 μm. C Phase-contrast images of cultured chondrocytes. Scale bars: 200 μm. D Average fold-change of in vitro cell expansion. Data shown as mean and SD (n = 13 individual donors). E In vitro differentiated JCC pellets. Photos show Safranin-O staining of P2 JCCs (top) and P9 JCCs (bottom). Bars: 500 µm. Right graph shows pellet size diameter measurements (n = 2). Error bars indicate SD. **p < 0.01 by Student’s t test.

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