Fig. 1: Isolation and characterization of mesenchymal stem cells. | npj Regenerative Medicine

Fig. 1: Isolation and characterization of mesenchymal stem cells.

From: Differential activation of Ca2+ influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration

Fig. 1

a Schematic showing isolation of mouse bone marrow cells (BMCs) and its properties of self-renewal and differentiation of MSCs. b Representative microscopic images displaying isolated bone marrow cells that show circular and round-shaped morphology of BMCs; whereas, adhered MSCs show fibroblast or eye-shaped morphology. c Immunofluorescence images of MSCs showing the expression of MSC-positive markers (CD29, CD44, and SCA1). d Immunophenotypic characterization of MSCs using flow cytometer showing the absolute counts of MSC positive markers such as CD29, CD44, and SCA1, as well as expression of MSC-negative (CD34, CD45, and CD11b). Isotype controls for each antibody were used as controls and FITC/rhodamine-conjugated secondary antibodies were used for their detection. e Bar graph (quantification from four independent experiments (expressed as mean ± standard error)) showing the percentage of cells expressing the MSC-positive and negative markers respectively. f, g Immunophenotypic characterization of BMCs using flow cytometer showing expression various markers (CD29, CD44, CD45, and CD11b). Bar graph expressed as mean ± standard error shows quantification of respective markers from three independent experiments.

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