Fig. 5: Impaired differentiation of Acvr1R206H/+ MuSCs in vivo. | npj Regenerative Medicine

Fig. 5: Impaired differentiation of Acvr1R206H/+ MuSCs in vivo.

From: Dynamics of skeletal muscle-resident stem cells during myogenesis in fibrodysplasia ossificans progressiva

Fig. 5

a Representative images of FACS-isolated MuSCs that were stained for the muscle stem cell marker Pax7 (green), the myogenic commitment marker MyoD (red), and nuclear DAPI (blue); a representative Pax7+MyoD+ cell is indicated (arrow). Scale bar = 50 µm. b Quantification of the percentage of muscle stem cells that are Pax7+MyoD− (quiescent; green bar), Pax7+MyoD+ (early differentiation; blue bar), and Pax7−MyoD+ (committed differentiation; red bar) from uninjured and injured muscles at 5 DPI. N ≥ 3 mice for each group; N > 100 MuSCs per timepoint and genotype were analyzed. Statistical significance was determined by two-way ANOVA. ***p < .0001; ns not significant. Color of statistics bars refers to groups being compared. c qRT-PCR expression of Id1 relative to Gapdh expression. *p < 0.05. Statistical significance was determined using an unpaired t-test. N = 2–3 mice per genotype per condition.

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