Fig. 1: Diaphragmatic-like construct components. | npj Regenerative Medicine

Fig. 1: Diaphragmatic-like construct components.

From: Customized bioreactor enables the production of 3D diaphragmatic constructs influencing matrix remodeling and fibroblast overgrowth

Fig. 1: Diaphragmatic-like construct components.

a Human skeletal muscle cells (hSkMC) characterization: immunofluorescence and quantification of cells positive for a proliferation marker (KI67) and myogenic markers (MYOD and MYOG; n = 4, mean ± s.d.). b Immunofluorescence of MHC and quantification of myogenic index (n = 4; mean ± s.d.). c Human fibroblasts characterization (hFb): immunofluorescence and quantification of TE7 and αSMA positive cells (n = 4). d Immunofluorescence and quantification of TE7-positive cells in 2D co-cultures of hSkMC (85%) and hFb (15%) after 4 days (n = 4; mean ± s.d.). e–j Immunofluorescence of ECM (Collagen type 1, Collagen type 4, Fibronectin, Laminin) and myogenic (Myosin heavy chain, Actin, sarcomeric Actinin 2) proteins on decellularized scaffolds. k Coverage rate of ECM proteins detected through mass spectrometry analysis (n = 3). Scale bars: 50 μm.

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