Fig. 1: Dedifferentiation and activation of Wnt/β-catenin signaling in reactive astrocytes after cerebral ischemia.
From: IRES-mediated Wnt2 translation in apoptotic neurons triggers astrocyte dedifferentiation
a, b Western-blotting and quantification of Nestin and Sox2 in ischemic cortex at different time points. *PNestin-1dpi = 0.031, *PNestin-3dpi = 0.033, **PNestin-7dpi = 0.0026, *PSox2-1dpi = 0.029, **PSox2-3dpi = 0.0031, *PSox2-7dpi = 0.028. One-way ANOVA followed by Bonferroni’s post hoc comparisons tests. N = 3 mice per time points. c Triple-immunostaining of Aldh1l1/Nestin/Sox2 in contralateral (Contra) and ipsilateral (Ipsi) side of ischemic cortex at 5 dpi. Bars = 50 μm. d Quantification of Sox2/Aldh1l1- and Nestin/Aldh1l1-positive cells in the zone expanded 200 μm from lesion border. N = 3 mice per group. e Triple-immunostaining of Nestin/GFAP/Sox2. Bar = 50 μm. f Quantification of Sox2/GFAP-positive cells and Nestin/GFAP-positive cells in the zone expanded 200 μm from lesion border. g, h Western-blotting and quantification of Axin2 and β-gal in ischemic cortex at different time points post injury. Notice the up-regulation of Axin2 and β-gal from 1 dpi. *PAxin2-1dpi = 0.019, **PAxin2-3dpi = 0.0024, *PAxin2-7dpi = 0.012, *PAxin2-14dpi = 0.035, *Pβgal-1dpi = 0.017, *Pβgal-3dpi = 0.021, *Pβgal-7dpi = 0.018, *Pβgal-14dpi = 0.027. One-way ANOVA followed by Bonferroni’s post hoc comparisons tests. N = 3 mice per time points. i Representative images of triple-immunostaining of Nestin/GFAP/β-gal and Sox2/GFAP/β-gal in Topgal mice at 5 dpi. Bar = 10 μm. Notice the induction of β-gal in Nestin- and Sox2-positive astrocytes in the injured cortex (Ipsi). Arrowheads point to triple-positive cells in (c, e, i). Mean ± standard error (SE). Asterisks indicate the comparison with control group. Asterisks with bars connecting two groups indicate difference between these two groups.
