Fig. 3: Deletion of Tjp2 in hepatocytes, but not in cholangiocytes, leads to a strong ductular reaction after DDC-diet. | npj Regenerative Medicine

Fig. 3: Deletion of Tjp2 in hepatocytes, but not in cholangiocytes, leads to a strong ductular reaction after DDC-diet.

From: ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver

Fig. 3: Deletion of Tjp2 in hepatocytes, but not in cholangiocytes, leads to a strong ductular reaction after DDC-diet.

a H&E, Ck19, and LacZ staining of liver sections from control and Tjp2 icKOCC mice after a 28-day DDC-diet. Scale bar: 100 μm. b Quantification of the Ck19-positive liver area. c Liver to body weight ratio for control and Tjp2 icKOHC mice after a 28-day DDC-diet. d H&E staining for control and Tjp2 icKOHC mice after a 28-day DDC-diet. Scale bar: 100 μm. e, f Ck19 staining showing periportal areas from control and Tjp2 icKOHC mice after a 28-day DDC diet and quantification from random liver sections. Scale bar: 100 μm. g, h Sox9 staining of liver sections from control and Tjp2 icKOHC mice after a 28-day DDC diet and quantification. Scale bar: 100 μm. i, j A6 staining of liver sections from control and Tjp2 icKOHC mice after a 28-day DDC diet and quantification. Scale bar: 100 μm. Data in (b, c, f, h, j) shown as mean ± SD, unpaired Student’s t test. *p < 0.05; **p < 0.005, ***p < 0.0005, ns = not significant (p > 0.05), with p < 0.05 considered a significant difference. (b): n = 6, (c, f): n = 7, (h, j): n-8 mice per cohort. icKOHH or icKOCC, tamoxifen-induced hepatocyte or cholangiocyte deletion, respectively.

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