Fig. 7: Inactivation of Yap/Taz but not Notch2 prevents the DDC-diet-induced hepatocyte to cholangiocyte transdifferentiation in Tjp2 icKOHC or Tjp2 cKO mice.
a, b LacZ staining of liver sections from the indicated mouse strains and quantification of LacZ-positive bile ducts. Scale bar: 100 μm. c Liver mRNA expression levels of Notch1, Notch2, and Jag1 of Tjp2 cKO mice fed a normal or DDC diet and normalized to that of controls. d Western blot analysis of Notch2 to confirm deletion in the Tjp2 Notch2 cKO liver. (tmi: transmembrane and intracellular domain, flL full-length). e, f Immunohistochemistry staining for Ck19 in liver sections from the indicated mouse lines and quantification. Scale bar: 100 μm. Data in (b, c, f) are shown as mean ± SD, unpaired Student’s t test. *p < 0.05; **p < 0.005, ***p < 0.0005, ns—not significant (p > 0.05), with p < 0.05 considered a significant difference. (b, f): n = 6, (c): n = 3 mice per cohort. icKOHH, tamoxifen-induced hepatocyte deletion.
