Fig. 4: Immune cells gene expression during pelvic floor muscle regeneration in non-irradiated and irradiated animals. | npj Regenerative Medicine

Fig. 4: Immune cells gene expression during pelvic floor muscle regeneration in non-irradiated and irradiated animals.

From: Muscle stem cells and fibro-adipogenic progenitors in female pelvic floor muscle regeneration following birth injury

Fig. 4

a Schematic representation of experimental design in panels B-H. b Graphical representation of the percentage of CD45+ total cells from all the sorted samples. c Graphical representation of the percentage of CD45+/CD4−/CD8− cells sorted for qPCR analysis. d Graphical representation of the % of CD4+ cells sorted for qPCR analysis. e Graphical representation of the percentage of CD8+ cells sorted for qPCR analysis. Dots represent single measurements; error bar represent SEM. Two-way ANOVA with Sidak’s post hoc. n = 3 animals. f qPCR analysis of pro-inflammatory genes (on the top) and of anti-inflammatory genes (on the bottom) for CD45+/CD4−/CD8− cells. g qPCR analysis of pro-inflammatory genes (on the top) and of anti-inflammatory genes (on the bottom) for CD4− cells. h qPCR analysis of pro-inflammatory genes (on the top) and of anti-inflammatory genes (on the bottom) for CD8− cells.

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