Fig. 3: ECCs can spontaneously differentiate into chondrocytes in vitro. | npj Regenerative Medicine

Fig. 3: ECCs can spontaneously differentiate into chondrocytes in vitro.

From: Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hPSCs

Fig. 3

a Gross appearance of ECCs seeded in a collagen sponge for 8 weeks. Scale bars, 1 mm. b Panoramic scanning showing the formation of cartilage masses in the collagen scaffold (inset: HE staining enlargement). Scale bars, 200 µm. (Inset) Scale bars, 50 µm. c–e The chondrocytes were positive for toluidine blue, alcian blue and safranin O staining. Scale bars, 100 µm. f–k Immunohistochemical staining showing that the chondrocytes express common chondrocytic markers, including collagen II, collagen X, aggrecan, RUNX2, collagen I, and the articular cartilage marker lubricin. Scale bars, 100 µm.

Back to article page