Fig. 2: Characterization of human USCs. | npj Regenerative Medicine

Fig. 2: Characterization of human USCs.

From: Injectable decellularized cartilage matrix hydrogel encapsulating urine-derived stem cells for immunomodulatory and cartilage defect regeneration

Fig. 2: Characterization of human USCs.The alternative text for this image may have been generated using AI.

a Morphology and proliferation of the USCs. Scale bars = 100 and 200 μm. b Representative images of osteogenic-induced (by ALP and Alizarin Red staining) and adipogenic-induced (by Oil red O staining) with USCs. Scale bar = 200 μm. c The potential for chondrogenic differentiation of the USCs after 28 days of 3D chondrogenic differentiation in vitro: (A) Gross appearance; (B) H&E staining; (C) safranin O staining; (D) Alcian blue; (E) aggrecan; (F) collagen II. Scale bar = 200 μm. e The mRNA expression of chondrogenesis-related genes (Aggrecan, Sox9, and Collagen II) was quantified in the USCs after 28 days of culture. f–h Expression of surface markers of the USCs. DMEM (standard culture medium); CM (chondrogenic induction medium). ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, * is the statistical difference compare with DMEM group. One-way ANOVA followed by the Tukey post hoc test was used. Each data point represented an average ± standard deviation, n = 4.

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