Fig. 2: NTRs are upregulated in MECs post-IR. | npj Regenerative Medicine

Fig. 2: NTRs are upregulated in MECs post-IR.

From: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells

Fig. 2: NTRs are upregulated in MECs post-IR.

A Box plots showing the median expression of neurotrophin signaling genes in bulk-RNAseq data from human salivary glands (n = 7 SMG, n = 6 SMG-IR, n = 6 PG, n = 5 PG-IR). The box represents the interquartile range and the bars span the minimum and maximum values. Star denotes statistical significance (p-value < 0.05 and fold change > 2, EdgeR-DeSeq2-Limma pipeline). B qPCR analysis of selected genes in irradiated human SMG and PG samples (n = 4 per group). Gene expression was normalized to GAPDH and non-irradiated controls. Statistical significance (p-value < 0.05) was determined by two-tailed t-test with log-transformed fold changes and is shown with a star above bars. Error bars represent standard error of the mean (SEM). C UMAP of scRNAseq from human SG from the Tabula Sapiens. D Balloon plot of expression of IPA’s upstream regulator and neurotrophin signaling genes in Tabula Sapiens. Color scale represents average scaled expression and size reflects percentage of cells expressing a given gene. E Number of IPA’s upstream regulator genes expressed in each cell type. F, G Immunofluorescence staining for NGFR and neurotrophin receptors (green) in control (A) and IR (B) human SMG. Smooth muscle actin (SMA, red) labels myoepithelial cells, and nerve-specific tubulin beta 3 (TUBB3, white) labels peripheral nerves. Scale bar = 50 μm.

Back to article page