Fig. 6: Dysregulation of neurotrophin receptors in MECs is associated with abnormal MEC differentiation and morphology. | npj Regenerative Medicine

Fig. 6: Dysregulation of neurotrophin receptors in MECs is associated with abnormal MEC differentiation and morphology.

From: Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells

Fig. 6: Dysregulation of neurotrophin receptors in MECs is associated with abnormal MEC differentiation and morphology.

A Experimental setup of murine MEC culture treated with exogenous NGF or RO for 48 h. PCR results showing fold change gene expression of selected MEC and duct genes 48 h post-treatment with NGF or RO (n = 4 per group). Statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001; Unpaired t-test compared to each control). B Immunostaining of MEC culture treated with RO for 48 h, with antibodies to NGF, neurotrophin receptors, KRT14 and KRT19 (all Green). All cultures were stained with SMA (red) and DAPI (blue). Scale bar = 150 µm. C Relative quantitation of MEC immunostaining from B, normalized to nuclei staining. Data is shown as mean +/− SEM. Two-tailed unpaired t-test was used. D Summary of NGF signaling in MEC culture.

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