Fig. 1: Depletion of macrophage Dnmt1 accelerates cutaneous wound healing and facilitates macrophage recruitment.

A Representative photographs of the wounds in Dnmt1^KO and Dnmt1^fl/fl mice on day 0, 3, 6, 9, and 12 post-injury. Scale bar, 1 mm. B Quantification of wound closure in (A). The wound areas at day 0 were set as 100%, and the closure rate represents the current wound area relative to the original area (n = 7 for Dnmt1^fl/fl mice, n = 8 for Dnmt1^KO mice). C Immunofluorescence staining to indicate macrophage infiltration in the wounds on day 0 and day 6. Red, F4/80⁺ macrophage; Green, DNMT1; Blue, the nucleus. Scale bar, 50 μm. D Statistics of the ratio of the number of F4/80⁺ cells in each field to the total number of cells (DAPI). Each dot represents the average ratio from five fields of view for each mouse. E Representative photographs of the wounds in Dnmt1^KO and Dnmt1^fl/fl mice that have been pretreated with LPS, on day 0, 3, 6, 9, and 12 post-injury. Scale bar, 1 mm. F Quantification of wound closure in (E) (n = 8 for Dnmt1^fl/fl mice, n = 9 for Dnmt1^KO mice). G Immunofluorescence staining to indicate macrophage infiltration in the wounds on day 6. Red, F4/80⁺ macrophage; Green, DNMT1; Blue, the nucleus. Scale bar, 50 μm. H, I Masson (H) and H&E (I) staining of the wounds from (G). Scale bar, 100 μm for (H), 200 μm for (I). J Representative photographs of the wounds in fat Dnmt1^KO and Dnmt1^fl/fl mice on day 0, 3, 6, 9, and 12 post-injury. Scale bar, 1 mm. K Quantification of wound closure in (J) (n = 10 for each group). L–N Immunofluorescence (L), Masson (M), and H&E (N) staining of the wounds from (J) on day 6. Red, F4/80⁺ macrophage; Green, DNMT1; Blue, the nucleus. Scale bar, 50 μm for (L), 100 μm for (M), 200 μm for (N). Data are presented as mean ± SEM. *P < 0.05 by unpaired Student’s t-test, or two-way ANOVA followed by Sidak’s post hoc test.