Fig. 4: Inhibition of Dnmt1 ameliorates the LPS-induced cholesterol accumulation. | npj Regenerative Medicine

Fig. 4: Inhibition of Dnmt1 ameliorates the LPS-induced cholesterol accumulation.

From: DNA methyltransferase 1 deficiency improves macrophage motility and wound healing by ameliorating cholesterol accumulation

Fig. 4

A Filipin staining of free cholesterol in RAW 264.7 cells (n = 3). The cells have been sequentially treated with 5-Aza/DMSO and LPS/PBS. Scale bar, 50 μm. B The total cholesterol content of RAW264.7 cells that were sequentially treated with 5-Aza/DMSO and LPS/PBS (n = 3). C The total cholesterol content of peritoneal macrophages (treated with LPS/PBS) from Dnmt1KO and Dnmt1fl/fl mice (n = 6). D Live-cell fluorescence images showing NBD-labeled cholesterol uptake by peritoneal macrophages (treated with LPS/PBS) from Dnmt1KO and Dnmt1fl/fl mice. The mean fluorescence intensity was obtained for the average of five fields in independent experiment (n = 4). Scale bar, 50 μm for upper two lines and 10 μm for lower two lines. E The total cholesterol content of RAW264.7 cells that were sequentially treated with 5-Aza/DMSO and LPS/PBS in serum-free media (n = 4). Data are presented as mean ± SEM. *P < 0.05 by one-way ANOVA followed by Turkey’s post hoc test.

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