Fig. 3: Histological analysis to investigate new bone formation stained with methylene blue/acid fuchsin.
From: 3D printing titanium grid scaffold facilitates osteogenesis in mandibular segmental defects
The calcified bone was stained a bright pink color, the Ti-mesh scaffold was stained black, and the bone substitute was stained brown in the slides (blue triangles), Havers’ systems were observed at high magnification (green triangles). a Histological section observation of Group 1 (low magnification). Scale bar, 2 mm. b Histological section observation of Group 2 (low magnification). Scale bar, 5 mm. c Histological section observation of Group 1 (high magnification). Scale bar, 200 µm. d Histological section observation of Group 2 (high magnification). Scale bar, 200 µm. e 1. Comparison of BV/TV in groups 1 and 2. e 2. Comparison of %Tb.Ar in groups 1 and 2. e 3. Comparison of BV/TV at the mesial, middle, and distal areas in groups 1 and 2. e 4. Comparison of %Tb.Ar at the mesial, middle, and distal area in groups 1 and 2 (*P < 0.05 compared to Group 1; #P < 0.05 compared with mesial area in Group 1; ※P < 0.05 compared with middle area in Group 1; ☆P < 0.05 compared with mesial area in Group 2; ΔP < 0.05 compared with middle area in Group 2).
