Fig. 6: LCN2 is upregulated in human brains with AD. | npj Regenerative Medicine

Fig. 6: LCN2 is upregulated in human brains with AD.

From: Nerve growth factor receptor (Ngfr) induces neurogenic plasticity by suppressing reactive astroglial Lcn2/Slc22a17 signaling in Alzheimer’s disease

Fig. 6: LCN2 is upregulated in human brains with AD.

Immunohistochemical stainings for LCN2 and GFAP on hippocampal brain sections of healthy control (a, b) and CERAD score C AD patient (c, d). Black-white insets (a1–d2) indicate individual fluorescent channels. LCN2 (top) and GFAP (bottom). e Quantification of LCN2-positive GFAP cells normalized to total GFAP cells. In total n = 7 human brains, n = 21 images analyzed. ***p < 0.001. f Immunostaining for GFAP on primary human astrocytes in 3D hydrogels: control and LCN2-treated. g Quantification of the volume of GFAP normalized to total number of GFAP cells. LCN2-treatment reduces the volume of astroglia, indicative or reactive states. Scale bars equal 50 μm. h GFAP and MAP2 immunostaining on control and LCN2-treated 3D hydrogel cultures of human primary fetal astroglia. i Quantification of normalized GFAP and MAP2-positive cells after the culture period. Scale bars equal 25 μm. Error bars represent standard error of the means.

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