Fig. 8: Restoration of the human fetal scalp cells to form CMU after environmental reprogramming.

Epidermal and dermal cells were harvested from the 24-week human fetal scalp with the same culture condition as the mouse skin explant culture. a Immunostaining and quantification of P63 and Klk7 expression show that inhibition of PKR or PKC results in decreased epidermal cell differentiation in human fetal scalp cell cultures at D3 compared to the controls. **p < 0.01. N = 8. b Immunostaining and quantification of Laminin and Collagen I expression show that addition of Tgfb led to a more complete basement membrane formation compared to the controls. **p < 0.01. N = 8. c Increased dermal cell attachment after the addition of VEGF recombinant protein compared to the controls. **p < 0.01. N = 8. d Hair regeneration after environmental reprogramming. Immunostaining for K14 and Collagen I which only stain human cells in the reconstituted skin. N = 4. e A comparing skin explant, wound-induced hair regeneration (WIHN), and normal developing skin shows that all of them have to form the competent morphogenetic field (CMF) before Turing patterning can take place. They may show different molecular paths but similar principles were used. See references in supplementary information.