Fig. 2: Metabolic profiling reveals decreased AKA levels in IL4I1 knockout mice and MS clinical samples.
From: Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration
a Schematic of neutral red method isolating lesions for mass spectrometry. b Heatmap of amino acids and metabolites from un-lesioned tissues, WT control lesions, and IL4I1-KO lesions. c Quantification of phenylpyruvic acid (PPA), indole-3-pyruvic acid (IPA), and 4-hydroxyphenylpyruvic acid (HPPA) in un-lesioned tissues (n = 4 mice) and WT control lesions (n = 10 mice) at 10 dpl (Welch’s t test). d Quantification of PPA, IPA, and HPPA in WT control lesions (n = 10 mice) and IL4I1-KO lesions (n = 10 mice) at 10 dpl (Welch’s t test). e Box plots of PPA and HPPA metabolism in healthy controls (HC, n = 79) and people with multiple sclerosis (MS, n = 279). f Box plots of PPA and HPPA metabolism in age-matched people with relapsing-remitting MS (RRMS, n = 81) and progressive MS (PMS, n = 81). The upper limit of the box represents 75th percentile, the lower limit of the box represents 25th percentile and the central line represents median, while whiskers represent 1.5 times the interquartile range. Each point represents an individual value. *P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant.
